Epidermal growth factor receptor relocalization and kinase activity are necessary for directional migration of keratinoc...
Published in Journal of Cell Science
Involucrin-positive keratinocytes demonstrate decreased migration speed but sustained directional migration in a DC elec...
Published in Journal of Investigative Dermatology Symposium Proceedings
Migration of human keratinocytes in electric fields requires growth factors and extracellular calcium
Published in Journal of Investigative Dermatology Symposium Proceedings
Intracellular calcium oscillations in cell populations of ras-transfected I-7 subline of human HaCaT keratinocytes
Published in Journal of Investigative Dermatology Symposium Proceedings
We have observed oscillations of intracellular Ca2+ (Ca[i]) concentration in populations of ras-transfected HaCaT keratinocytes of I-7 subline. In postconfluent monolayers of I-7 keratinocytes, an increase in extracellular Ca2+ (Ca[o]) concentration to 0.25-0.5 mM induced sinusoidal Ca(i) oscillations, which persisted longer than 1 h with amplitude...
Thapsigargin induces phosphorylation of the 27-kDa heat shock protein in human keratinocytes
Published in Journal of Investigative Dermatology Symposium Proceedings
In the human keratinocyte line HaCaT, the nonphosphorylated 27-kDa heat shock protein (HSP27) isoform A (pI 6.5) is constitutively expressed. Application of thapsigargin, which inhibits Ca2+-ATPase in the endoplasmic reticulum, results in the rapid formation of the phosphorylated HSP27 isoform B (pI 6.0) and reduction of HSP27 A without affecting t...
Human keratinocytes migrate to the negative pole in direct current electric fields comparable to those measured in mamma...
Published in Journal of Cell Science
Previous measurements of the lateral electric fields near skin wounds in guinea pigs have detected DC fields between 100-200 mV/mm near the edge of the wound. We have studied the translocation response of motile primary human keratinocytes migrating on a collagen substrate while exposed to similar physiological DC electric fields. We find that kera...
Imposition of a physiologic DC electric field alters the migratory response of human keratinocytes on extracellular matr...
Published in Journal of Investigative Dermatology Symposium Proceedings
Outwardly directed ionic currents have been measured leaving skin wounds in vivo. These currents generate physiologic electric fields of approximately 100 mV/mm, which may function to direct keratinocyte migration toward the healing wound. We investigated whether the substrate on which the keratinocyte migrates modulates the galvanotactic response ...
