Dynamics of neutrophil infiltration during cutaneous wound healing and infection using fluorescence imaging
Published in Journal of Investigative Dermatology Symposium Proceedings
Published in Journal of Investigative Dermatology Symposium Proceedings
Published in Journal of Investigative Dermatology Symposium Proceedings
Published in Journal of Investigative Dermatology Symposium Proceedings
Published in Journal of Investigative Dermatology Symposium Proceedings
In the human keratinocyte line HaCaT, the nonphosphorylated 27-kDa heat shock protein (HSP27) isoform A (pI 6.5) is constitutively expressed. Application of thapsigargin, which inhibits Ca2+-ATPase in the endoplasmic reticulum, results in the rapid formation of the phosphorylated HSP27 isoform B (pI 6.0) and reduction of HSP27 A without affecting t...
Published in Journal of Investigative Dermatology Symposium Proceedings
Published in Journal of Investigative Dermatology Symposium Proceedings
Published in Journal of Investigative Dermatology Symposium Proceedings
We have observed oscillations of intracellular Ca2+ (Ca[i]) concentration in populations of ras-transfected HaCaT keratinocytes of I-7 subline. In postconfluent monolayers of I-7 keratinocytes, an increase in extracellular Ca2+ (Ca[o]) concentration to 0.25-0.5 mM induced sinusoidal Ca(i) oscillations, which persisted longer than 1 h with amplitude...
Published in Journal of Investigative Dermatology Symposium Proceedings
Published in Journal of Investigative Dermatology Symposium Proceedings
Outwardly directed ionic currents have been measured leaving skin wounds in vivo. These currents generate physiologic electric fields of approximately 100 mV/mm, which may function to direct keratinocyte migration toward the healing wound. We investigated whether the substrate on which the keratinocyte migrates modulates the galvanotactic response ...
Published in Journal of Investigative Dermatology Symposium Proceedings